Journal: Drug Design, Development and Therapy
Article Title: Effect of Berberine on Hyperuricemia and Kidney Injury: A Network Pharmacology Analysis and Experimental Validation in a Mouse Model
doi: 10.2147/DDDT.S317776
Figure Lengend Snippet: BBR improved UA excretion by regulating the mRNA and protein expression of URAT1. ( A ) Kidney immunohistochemical staining (400×) and ( B ) representative Western blots of URAT1. Quantitative analysis of ( C ) immunohistochemical staining and ( D ) Western blots results (n=3). ( E ) mRNA expression level of URAT1 (n=4). ( F ) Initial orientation of the BBR in the URAT1 active pocket. ( G ) Chemical interactions between BBR and URAT1. All the results are shown as mean ± SEM.
Article Snippet: Primary antibodies against NLRP3, IL-1β and β-actin were obtained from Abcam (Cambridge, UK), primary antibodies against ASC and caspase1 were obtained from Biosciences (Cincinnati, OH, USA), and primary antibodies against URAT1 was obtained from Proteintech (Wuhan, China).
Techniques: Expressing, Immunohistochemical staining, Staining, Western Blot